diff --git a/PyNutilGUI.py b/PyNutilGUI.py
index 93dce269baa3618287313ad2f1487aee8146ed3b..0db7f7aa06c37964b24486082181d78efe14b2ba 100644
--- a/PyNutilGUI.py
+++ b/PyNutilGUI.py
@@ -12,6 +12,10 @@ from tkinter import colorchooser
 root = Tk()
 #root.geometry("300x300")
 root.title("PyNutil")
+root.wm_iconbitmap("Logo_PyNutil.ico")
+#photo = tkinter.PhotoImage(file = 'Logo_PyNutil.ico')
+#root.wm_iconphoto(False, photo)
+
 arguments = {
    "registration_json":None,
    "object_colour":None
@@ -27,9 +31,6 @@ selected_directory = StringVar(value="directory")
 colour = ["colour","black","red","blue","green"]
 selected_colour = StringVar(value=colour[0])
 
-# root.wm_iconbitmap("Logo_PyNutil.ico")
-#photo = tkinter.PhotoImage(file = 'Logo_PyNutil.ico')
-#root.wm_iconphoto(False, photo)
 def donothing():
    filewin = Toplevel(root)
    label = Label(filewin, text="Do nothing")
@@ -39,7 +40,20 @@ def about_pynutil():
    filewin = Toplevel(root)
    label = Label(filewin, text="PyNutil is an application for brain-wide mapping using a reference brain atlas")
    label.pack()
- 
+   
+def open_registration_json():
+   value = askopenfilename()
+   arguments["registration_json"] = value
+   print(arguments)
+
+def choose_colour():
+   value = colorchooser.askcolor()
+   arguments["object_colour"] = value
+   print(list(value[0]))
+
+def start_analysis():
+   #your code here
+   return
 
 #Creating a menu
 root.option_add('*tearOff', FALSE)
@@ -59,57 +73,51 @@ menu_file.add_command(label='New', command=donothing)
 menu_file.add_command(label='Exit', command=root.quit)
 menu_help.add_command(label='About PyNutil', command=about_pynutil)
 
-
 #Creating a content frame"
 mainframe = ttk.Frame(root, padding="12 12 12 12") # left top right bottom
 mainframe.grid(column=0, row=0, sticky=(N, W, E, S))
 root.columnconfigure(0, weight=1) # column to expand if there is extra space
 root.rowconfigure(0, weight=1) # row to expand if there is extra space
 
-#ttk.Label(mainframe, text="PyNutil settings:").grid(column=0, row=1, sticky=W)
-ttk.Label(mainframe, text="Select reference atlas:", width=30).grid(column=1, row=1, sticky=W)
-ttk.Label(mainframe, text="Select registration JSON:", width=30).grid(column=1, row=2, sticky=W)
-ttk.Label(mainframe, text="Select segmentation folder:", width=30).grid(column=1, row=3, sticky=W)
-ttk.Label(mainframe, text="Select object colour:", width=30).grid(column=1, row=4, sticky=W)
-ttk.Label(mainframe, text="Select output directory:", width=30).grid(column=1, row=5, sticky=W)
-ttk.Label(mainframe, text="Start analysis:", width=30).grid(column=1, row=6, sticky=W)
-
-ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=3, row=1, sticky=W)
-ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=3, row=2, sticky=W)
-ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=3, row=3, sticky=W)
-ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=3, row=4, sticky=W)
-ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=3, row=5, sticky=W)
-ttk.Button(mainframe, text="Docs", width=8, command="buttonpressed").grid(column=3, row=6, sticky=W)
-
-def open_registration_json():
-   value = askopenfilename()
-   arguments["registration_json"] = value
-   print(arguments)
-
-def choose_colour():
-   value = colorchooser.askcolor()
-   arguments["object_colour"] = value
-   print(list(value[0]))
-
-def start_analysis():
-   #your code here
-   return
-
-
+#Creating a content frame"
+bottomframe = ttk.Frame(root, padding="12 12 12 12") # left top right bottom
+mainframe.grid(column=0, row=0, sticky=(N, W, E, S))
+root.columnconfigure(0, weight=1) # column to expand if there is extra space
+root.rowconfigure(0, weight=1) # row to expand if there is extra space
 
-ttk.OptionMenu(mainframe, selected_atlas, "Reference Atlas", *atlas).grid(column=2, row=1, sticky=W)
-#ttk.OptionMenu(mainframe, selected_directory, *directory).grid(column=3, row=2, sticky=W)
-#ttk.OptionMenu(mainframe, selected_directory, *directory).grid(column=3, row=3, sticky=W)
-#ttk.OptionMenu(mainframe, selected_colour, *colour).grid(column=3, row=4, sticky=W)
-#ttk.OptionMenu(mainframe, selected_directory, *directory).grid(column=3, row=5, sticky=W)
+# Select reference atlas
+ttk.Label(mainframe, text="Select reference atlas:", width=25).grid(column=1, row=1, sticky=W)
+ttk.OptionMenu(mainframe, selected_atlas, "Reference Atlas", *atlas).grid(column=2, row=1, columnspan=2)
+ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=4, row=1, sticky=W)
 
-#ttk.Button(mainframe, width=16, text="Browse...", command="buttonpressed").grid(column=4, row=1, sticky=W)
+#Select registration JSON
+ttk.Label(mainframe, text="Select registration JSON:", width=25).grid(column=1, row=2, sticky=W)
 ttk.Button(mainframe, width=16, text="Browse...", command=open_registration_json).grid(column=2, row=2, sticky=W)
+Text(mainframe, height = 1, width =40).grid(column=3, row=2, sticky= W)
+ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=4, row=2, sticky=W)
 
+#Select segmentation folder
+ttk.Label(mainframe, text="Select segmentation folder:", width=25).grid(column=1, row=3, sticky=W)
 ttk.Button(mainframe, width=16, text="Browse...", command="buttonpressed").grid(column=2, row=3, sticky=W)
+Text(mainframe, height = 1, width =40).grid(column=3, row=3, sticky= W)
+ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=4, row=3, sticky=W)
+
+#Select object colour
+ttk.Label(mainframe, text="Select object colour:", width=25).grid(column=1, row=4, sticky=W)
 ttk.Button(mainframe, width=16, text="Colour", command=choose_colour).grid(column=2, row=4, sticky=W)
+Text(mainframe, height = 1, width =40).grid(column=3, row=4, sticky= W)
+ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=4, row=4, sticky=W)
+
+#Select output directory
+ttk.Label(mainframe, text="Select output directory:", width=25).grid(column=1, row=5, sticky=W)
 ttk.Button(mainframe, width=16, text="Browse...", command="buttonpressed").grid(column=2, row=5, sticky=W)
-ttk.Button(mainframe, width=16, text="Run", command="buttonpressed").grid(column= 2, row=6, sticky=W)
+Text(mainframe, height = 1, width =40).grid(column=3, row=5, sticky= W)
+ttk.Button(mainframe, text="Help", width=8, command="buttonpressed").grid(column=4, row=5, sticky=W)
+
+#Start analysis
+ttk.Label(mainframe, text="Start analysis:", width=25).grid(column=1, row=6, sticky=W)
+ttk.Button(mainframe, width = 52, text="Run", command="buttonpressed").grid(column= 3, row=6)
+ttk.Button(mainframe, text="Docs", width=8, command="buttonpressed").grid(column=4, row=6, sticky=W)
 
 # sunken frame around mainframe
 """